OneWorld Biotech, Inc. is now pleased to offer expanded and cost effective NucleoWayTM Molecular Cloning services to the wider scientific community. We have been helping biotech and pharma clients with custom gene cloning and vector construction, RNA and mRNA synthesis, plasmid DNA and RNA purification, and other DNA/RNA analysis projects over the past nine years.
Cloning by Restriction Enzyme Digestion: Digest plasmid containing the insert, gel isolate insert, ligate to vector at same sites or at blunt ends.
Cloning by PCR Amplification: PCR amplify insert, digest with restriction enzymes, and ligate to vector at same sites or clone by TA or blunt end cloning.
Cloning by Gene Synthesis: Clone synthetic gene or sequence using restriction enzyme sites or by TA or blunt end cloning.
Sequence and Ligase Independent Cloning: Amplify gene using special PCR primers and anneal insert and linear vector.
Cloning by Gibson Assembly: Use exonuclease-based method to seamlessly assemble several DNA fragments in correct order for gene synthesis, synthetic biology, and novel expression vectors.
Cloning by RT-PCR: Isolate RNA/mRNA from cells and tissues, reverse transcribe RNA using gene specific primers and clone into any plasmid.
Protein Expression Vectors: Vectors created by cloning genes, marker genes, chimeric genes, fusion genes, splice variants, mutants, epitope and purification tags, promoters, chimeric promoters, terminators, antibiotic resistance genes, insulators, and MARS to create vectors for transient and stable expression in E. coli, yeast, insect, and mammalian cells.
Viral Expression Vectors: AAV, Adeno, Lenti, Baculovirus, and other viral vectors with combination of genes, promoters and tags.
Gene Targeting Vectors: Vectors for homologous recombination, knock-in, knock-out, Cre, FLP, and Flox constructs for genome integration.
Inducible Expression Vectors: Conditional and inducible systems for a temporal and spatial controlled activation of genes including Tet-OnTM, Tet-OffTM, GeneSwitch±, Ecdysone-inducible, and tamoxifen-inducible.
Small RNA Vectors: Clone siRNA, miRNA and shRNA in plasmids with pol II or pol III promoters for transient and stable expression.
We are a truly custom services company and our services can be tailored to meet your specific requirements. We can fulfill your needs of recombinant DNA constructs and other molecular biology experiments including DNA- and RNA-based assays and analyses. To learn more about how we can accelerate your research and to obtain a no-obligation quote, contact us today.
We offer a comprehensive set of RNA services and have the flexibility to create specialized methods for unique scientific objectives.
Our RNA services are described below in general terms. Working with OneWorld Biotech, you will experience first-hand our commitment to exceptional service.
OneWorld Biotech’s comprehensive synthesis services allow us to use the latest technologies to provide clients with the highest quality RNA using T7, T3, SP6 RNA polymerases, RNA with homogenous 5’ and 3’ ends, RNA produced in E. coli, and mRNA with 5’ cap and 3’ poly (A) tail. RNAs of 20 to 5000 nucleotides with synthesis scale of 100 ug to 100 mg are available. Projects are tailored to meet client specifications.
Our goal is to exceed our client’s expectations with respect to the quality of work. We employ the Quality by Design approach at all steps of synthesis and purification. Quantity (NanoDrop 2000, Fluorometric), purity (NanoDrop 2000, denaturing gel electrophoresis, HPLC), and integrity (Agilent Bioanalyzer, denaturing gel electrophoresis) are evaluated and QC data is shared with our clients.
The success of all of gene expression analysis methods from Northern blotting to RT-PCR and RNAseq is dependent on the quality of RNA isolated. We can assist with your gene expression analysis research by providing high quality RNA as well as full-length cDNA prepared for optimum performance in analysis. RNA, poly(A) RNA, and microRNA isolation from all types of cells and tissues, including demanding samples such as formalin-fixed and paraffin-embedded tissues (FFPE) are prepared to the highest standards, and to whatever scale required.
RNA extracted from cells and tissues using industry standard reagents and in-house expertise has been used for gene expression analysis, cDNA library construction and screening in HEK293 cells to identify a drug target, and cloning of low abundance RNAs by reverse transcription.