OneWorld Biotech’s ProteoWay™ Protein Production Optimization ensures that each stage of the protein expression process — from vector design, host selection, culture conditions and purification to protein characterization — delivers the highest quality biologically active protein possible.
From antibodies and GPCRs to proteases, lipases and kinases, our platform delivers consistent and highest quality of biologically proteins in their native state. Let OneWorld Biotech help you do it the right way, the successful way….. The ProteoWay™!
Researchers are constantly challenged by the need for highly pure proteins in R&D and product development. Novel approaches to meet the demands of tomorrow’s products are constantly being developed. Each expression platform has its own merits and disadvantages, and one may be more suitable for a particular protein than others. We narrow the choices by considering many factors to select the optimal vector and host combination.The success rate for heterologous gene expression and protein purification is greatly increased by optimizing each process step: vector design, transient vs. stable expression, inducible vs. constitutive, need for affinity tags, and choice of host system. The construct design also depends on whether the protein will be used for functional studies, antibody development, assay development, structural studies, diagnostic tests, or therapeutics.
Transient Expression in Mammalian Cells (HEK293, CHO, HeLa and others)
Transient Expression in Insect Cells (Sf9, Sf21, High Five, and others)
Stable Expression in Mammalian Cells and Selection of Pools
Stable Expression in Mammalian Cells and Clonal Selection
Stable Expression in Mammalian Cells, Clonal Selection and Gene Amplification
Stable Expression in Insect Cells and Selection of Pools or Clones
E. coli Expression
See below for additional information on protein expression and purification services.
Mammalian cell biotechnology is a demanding system for the production of recombinant proteins compared to microbial systems. We employ recent developments – genetic, biochemical, metabolic control of cells, glycosylation, bioreactors properties, and new chromatography solutions – to reduce the time and cost required to establish transient and stable eukaryotic cell-based protein production.
We offer three types of protein expression in insect cells: 1) stable transformation of insect cells for long term continuous production of difficult-to-express cytoplasmic, membrane, nuclear, and secretary proteins, 2) plasmid-based transient transfection for quick purification of protein variants, and 3) Baculovirus expression vector system with transfer vectors for multi-host expression in E. coli, insect, and mammalian cells.
Our experience with a variety of expression systems and our know-how enables us to make the right microbial host choice that meets your needs. We are experts in optimizing protein expression using established and new developments in tunable expression, solubility technology, and protein targeting to maximize protein yield, biological activity and purity.
We are the one-stop solution for your antibody projects. We offer a range of production and purification services for hybridoma culture and recombinant antibody production heterologous system (bacteria, yeast, or mammalian cells), and phage display.
Our in-house protein purification process and techniques are customized to a wide variety of expression hosts from small-scale to large-scale production of recombinant proteins. For each protein, we evaluate a combination of purification methods for simplicity and cost to obtain highly pure and biologically active protein.
All proteins are subject to quality control during and after purification. OneWorld Biotech’s ability to quickly, routinely, and comprehensively characterize proteins accelerates project completion and assures product quality.